We investigate post-transcriptional gene regulation in flies and mammals by dissecting  the molecular principles and timescales of RNA decay and their biological and biomedical implications. To this end, we employ quantitative biochemical methods, cell culture experiments, and in vivo genetics to dissect the mechanisms and biological functions of RNA silencing in flies and cultured mammalian cells; and we combine time-resolved transcriptomics and functional genetics with bioinformatics, to unravel the time-scales and functional organization of post-transcriptional gene silencing at the molecular and genomic scale.


Publications

Reichholf B, Herzog VA, Fasching N, Manzenreither RA, Sowemimo I, Ameres SL. 2019. Time-resolved small RNA sequencing unravels the molecular principles of microRNA homeostasis. Mol Cell. 75(4): 756-768, doi: 10.1016/j.molcel.2019.06.018

Herzog VA, Reichholf B, Neumann T, Rescheneder P, Bhat P, Burkard TR, Wlotzka W, von Haeseler A, Zuber J, Ameres SL. 2017. Thiol-linked alkylation to assess expression dynamics. Nat Methods. 14(12):1198-1204. doi: 10.1038/nmeth.4435.

Reimão-Pinto MM, Manzenreither RA, Burkard TR, Sledz P, Jinek M, Mechtler K,  Ameres SL. 2016. Molecular basis for cytoplasmic RNA turnover by uridylation-triggered RNA decay in Drosophila. EMBO J. 35(22):2417-2434. 10.15252/embj.201695164


Phone office
+43 1 4277 74821


Phone lab
+43 1 4277-743-22/23/24/25


E-mail:
Stefan.ameres@univie.ac.at


Address:
EMBO Young Investigator
Group Leader at the Max Perutz Labs
Campus Vienna Biocenter
Dr. Bohr-Gasse 9,
A-1030 Vienna,
Austria